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STEMCELL Technologies Inc easyseptm human t cell isolation kit
Easyseptm Human T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easyseptm+human+t+cell+isolation+kit/easysep+human+t+cell+isolation+kit/pm40649953-180-15-18
Average 90 stars, based on 1 article reviews
easyseptm human t cell isolation kit - by Bioz Stars, 2026-11
90/100 stars

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Article Title: IL-36 signaling as a drug target in Crohn’s disease patients with IL36RN mutations
Article Snippet: CD3 + and CD3 − cells were then separated using the EasySep Human T Cell Isolation Kit (Stemcell), following the manufacturer’s instructions.

Article Title: IL-36 signaling as a drug target in Crohn's disease patients with IL36RN mutations.
Article Snippet: Daily quality control checks were performed using Sphero Rainbow Calibration Particles (BD Biosciences).

Article Title: IL-36 signaling as a drug target in Crohn’s disease patients with IL36RN mutations
Article Snippet: EasySep Human T Cell Isolation Kit , Stemcell , 17951.

Isolation:

Article Title: Knockdown of TIM3 Hampers Dendritic Cell Maturation and Induces Immune Suppression by Modulating T-Cell Responses.
Article Snippet: .. T cells from the same donor were isolated using the EasySepTM Human T Cell Isolation Kit (Stemcell, Vancouver, BC, Canada). ..

Article Title: Disparate Roles of Cell-Cell Contact and Cytokine Secretion in an In Vitro Model of the Seminoma Microenvironment.
Article Snippet: .. The sorting of individual leukocyte subpopulations was achieved with the EasySepTM human T cell or Monocyte Isolation Kit (Stemcell Technologies, Cologne, Germany) according to the instructions of the manufacturer. ..

Article Title: Lipid nanoparticle formulations and compositions
Article Snippet: .. T cells were isolated from fresh leukopaks with EasySepTM Human T Cell Isolation Kit (#100-0695, STEMCELL Technologies) following manufacturer's manual. .. Briefly, a Leukopak was treated with ammonium chloride solution (#07800, STEMCELL Technologies) to lyse residual red blood cells and platelets and washed with EasySepTM Buffer (#20144, STEMCELL Technologies) with gentle centrifugations.

Article Title: Modeling host-microbe interactions in immunocompetent engineered human gut tissues
Article Snippet: .. T cells were isolated from PBMCs using the EasySepTM Human T Cell Isolation kit (17951, STEMCELL Technologies) and EasySepTM Magnet (18000, STEMCELL Technologies) following manufacturer instructions. .. Viability and number of immune cells was assessed with Trypan Blue (216040, Invitrogen) on a Countess II (Invitrogen).

Article Title: T cell toxicity induced by tigecycline binding to the mitochondrial ribosome.
Article Snippet: .. T cells were isolated using EasySepTM Human T Cell Isolation Kit (Stemcell Technologies). .. Purified T cells were counted and 85,000 cells per well seeded onto 96-well Ubottom plates with/without Human T-Activator CD3/CD28 beads (Invitrogen) at a 1:2.8 cell:bead ratio and 10 ng/ml IL-2 (Proteintech) in RPMI-1640 ( + 10% FBS, 2.05 mM L-glutamine (Sigma), and 55μM 2-ME (Gibco)) for 48 h. To assessmitochondrial translation, activated T cells werewashed twicewithmethionine/cysteine/pyruvate-freeDMEMand incubated in methionine-depletion medium (DMEM supplemented with 10% dialyzed FBS, 1× GlutaMAX, 1× sodium pyruvate, and 0.2 mM L-cystine) for 30min at 37 °C.

Cell Isolation:

Article Title: Knockdown of TIM3 Hampers Dendritic Cell Maturation and Induces Immune Suppression by Modulating T-Cell Responses.
Article Snippet: .. T cells from the same donor were isolated using the EasySepTM Human T Cell Isolation Kit (Stemcell, Vancouver, BC, Canada). ..

Article Title: Lipid nanoparticle formulations and compositions
Article Snippet: .. T cells were isolated from fresh leukopaks with EasySepTM Human T Cell Isolation Kit (#100-0695, STEMCELL Technologies) following manufacturer's manual. .. Briefly, a Leukopak was treated with ammonium chloride solution (#07800, STEMCELL Technologies) to lyse residual red blood cells and platelets and washed with EasySepTM Buffer (#20144, STEMCELL Technologies) with gentle centrifugations.

Article Title: Modeling host-microbe interactions in immunocompetent engineered human gut tissues
Article Snippet: .. T cells were isolated from PBMCs using the EasySepTM Human T Cell Isolation kit (17951, STEMCELL Technologies) and EasySepTM Magnet (18000, STEMCELL Technologies) following manufacturer instructions. .. Viability and number of immune cells was assessed with Trypan Blue (216040, Invitrogen) on a Countess II (Invitrogen).

Article Title: T cell toxicity induced by tigecycline binding to the mitochondrial ribosome.
Article Snippet: .. T cells were isolated using EasySepTM Human T Cell Isolation Kit (Stemcell Technologies). .. Purified T cells were counted and 85,000 cells per well seeded onto 96-well Ubottom plates with/without Human T-Activator CD3/CD28 beads (Invitrogen) at a 1:2.8 cell:bead ratio and 10 ng/ml IL-2 (Proteintech) in RPMI-1640 ( + 10% FBS, 2.05 mM L-glutamine (Sigma), and 55μM 2-ME (Gibco)) for 48 h. To assessmitochondrial translation, activated T cells werewashed twicewithmethionine/cysteine/pyruvate-freeDMEMand incubated in methionine-depletion medium (DMEM supplemented with 10% dialyzed FBS, 1× GlutaMAX, 1× sodium pyruvate, and 0.2 mM L-cystine) for 30min at 37 °C.



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